Figure 8: Characterization of DSEL mice alone and in combination with Gsα KO. | Nature Communications

Figure 8: Characterization of DSEL mice alone and in combination with Gsα KO.

From: G-protein stimulatory subunit alpha and Gq/11α G-proteins are both required to maintain quiescent stem-like chondrocytes

Figure 8

(a–d) DSEL mice9. No changes were observed in the growth plate height (a), BrdU incorporation (b), apoptosis (c) and BrdU-labelled stem-like cells co-visualized with TUNEL-positive cells (d, experimental details are the same as for Fig. 7a) between mice with one copy of mutated PTH receptor (DSEL mutation, D/+) and two copies of the mutated receptor (D/D). (e) In mice with inactivated Gsα in their growth plate, the DSEL mutation of two copies of the PTH receptor increased the number of cells positive for cleaved caspase-3 as compared with those in which only one copy of the PTH receptor was mutated. Tamoxifen was injected at 3 days of age and mice were analysed 9 days later. Scale bars, 100 μm.

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