Figure 1: The level of brain-specific miR-9 is transcriptionally and post-transcriptionally regulated during RA-induced neuronal differentiation of P19 cells.
From: Lin28a regulates neuronal differentiation and controls miR-9 production
(a,c,d) Real-time qRT–PCR analysis of mature miR-9, let-7a and miR-302a levels at different stages of RA-induced neuronal differentiation (Day—d). The values were normalized to miR-16 levels. The fold change was plotted relative to values derived from undifferentiated cells (d0), which were set to 1. Mean values and s.d. of three independent biological replicates are shown. (b) Real-time qRT–PCR of miR-9 primary transcripts (pri-miR) at different stages of RA-induced P19 cell neuronal differentiation. The fold changes of the corresponding pri-miRNA abundance, pri-miR-9-1 (black bars), pri-miR-9-2 (grey bars) and pri-miR-9-3 (white bars) were plotted relative to the d0 values, which were set to 1. The values were normalized to the cyclophilin A mRNA levels. Mean values and s.d. of three independent experiments are shown.
