Figure 3: The p97 segregase complex extracts DDB2 and XPC from chromatin. | Nature Communications

Figure 3: The p97 segregase complex extracts DDB2 and XPC from chromatin.

From: Chromatin retention of DNA damage sensors DDB2 and XPC through loss of p97 segregase causes genotoxicity

Figure 3

(a) Chromatin retention of DDB2 and XPC visualized 15 and 180 min after induction of UV damage spots in human cells treated with siRNA targeting p97 or non-coding (NC) control. Scale bar, 10 μm. (b,c) Quantification of DDB2 and XPC, respectively, in chromatin over three experiments (>300 nuclei). Error bars, s.e.m.; ***P<0.001 relative to siNC control (unpaired two-tailed t-test). (d) XPC hyperubiquitination and DDB2 degradation revealed by whole-cell immunoblot analysis after treatment with siRNA against p97 or NC control. (e) Levels of soluble (non-chromatin) and chromatin-bound DDB2 assessed in the respective cellular fractions.

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