Figure 5: An excess of damage sensors attenuates repair and causes genomic instability. | Nature Communications

Figure 5: An excess of damage sensors attenuates repair and causes genomic instability.

From: Chromatin retention of DNA damage sensors DDB2 and XPC through loss of p97 segregase causes genotoxicity

Figure 5

(a) Excision of 6-4PPs in human cells treated with siRNA targeting p97 or XPC, used as comparator inflicting a severe repair defect; siNC, non-coding RNA control. Error bars, s.e.m. (n=3, each experiment with four replicates), **P<0.01 (unpaired two-tailed t-test). (b) Excision of CPDs upon treatment with siRNA targeting p97 or DDB2 (n=3, each experiment with four replicates), *P<0.05 (unpaired two-tailed t-test). In the case of CPDs, depletion of DDB2 is sufficient to compromise excision efficiency. (c) Metaphase spreads showing a progressively increasing genomic instability. Representative chromosomes are magnified in the inserts. (d) Chromosomal aberrations in cells treated with siRNA targeting the indicated proteins (n=3, 60 cells in a blinded analysis). *P<0.05 relative to siNC and cells with double depletion (unpaired two-tailed t-test). (e) Chromosomal aberrations in cells overexpressing the indicated proteins (n=3, 60 cells in a blinded analysis). *P<0.05 relative to cells expressing FLAG only and the double overexpressing cells (unpaired two-tailed t-test).

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