Figure 1: Morphology of HCs in larval zebrafish retina. | Nature Communications

Figure 1: Morphology of HCs in larval zebrafish retina.

From: Transmission from the dominant input shapes the stereotypic ratio of photoreceptor inputs onto horizontal cells

Figure 1

(a) Examples of HCs at 3.5 d.p.f. visualized by injecting plasmid DNA (see Methods for details) into fertilized eggs. Shown here is the maximum intensity projection of a confocal image stack of the back of an isolated eye. (b,c) Orthogonal views of two-photon reconstructions of cone HCs in live fish (3.5 d.p.f.). Dendritic tips extending from the HCs are clearly visible from these side views. Scale bars (a–c), 5 μm. (d) Tip density plotted against dendritic field size of morphologically classified H1/2 and H3 HCs at 3.5 d.p.f. The dendritic field is defined as the area encompassed by a convex polygon whose corners touch the outer most dendritic tips. Each open or grey symbol represents one cell. Black symbols and error bars represent means and s.e.m. P-values from Wilcoxon–Mann–Whitney rank sum test. n=9 for H1/2 and n=6 for H3. (e) EM image of a cone pedicle showing invaginating HC dendritic tips (red) that are apposed to characteristic presynaptic ribbons, sites of transmitter release (arrows). (f) Single plane confocal image showing Gria2/3 (also known as GluR2/3) immunoreactive puncta (green) colocalized with an HC dendritic tip (red) within a cone pedicle (blue). Scale bar (e,f), 0.5 μm.

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