Figure 4: Dynamic H3 HC processes selectively target ultraviolet cones.

(a,b) Multiphoton time lapse imaging of an H3 HC in the background of (a) Tg(sws1:GFP) or (b) Tg(sws2:GFP). For each time point, an orthogonal view of the cell and labelled cones within the boxed region is provided below the connectivity map. A line scan of this region showing the relative pixel intensities of the two channels (magenta, ultraviolet cone signal or cyan, blue cone signal; yellow, HC signal) is presented below the view of the cell. Asterisks mark the location where, over time, a dendritic tip emerged and contacted ultraviolet or blue cones. Higher magnification of this location is shown on the right panels. A confocal reconstruction of the cell after fixation at the final time point (104 h.p.f.) clearly identifies the formation of new synapses. In the connectivity maps, solid circles represent synapses added in-between time points; open circles are stable contacts and X indicates eliminated contacts. Scale bars, 5 μm. (c) Population data showing the mean number of ultraviolet and blue cone synapses added and eliminated during the time course of multiphoton imaging. (d) Net change in the number of ultraviolet and blue cone synapses (n=5 for ultraviolet and n=3 for blue). Error bars are s.e.m.