Figure 1: Unc5C is expressed by callosally projecting Satb2-positive neurons and Satb2 regulates Unc5C expression.
From: Unc5C and DCC act downstream of Ctip2 and Satb2 and contribute to corpus callosum formation
(a) Unc5C mRNA expression in wild-type and Satb2−/−-mutant mice at E18.5. In sections of Satb2−/−-mutant brains, Unc5C continues to be expressed in only the subplate, but is absent in other cortical layers. (b) Unc5C expression in E14.5 wild-type embryos in cells that were electroporated at E12.5 with Satb2/Ski/EGFP or EGFP control. GFP IHC depicts the region electroporated. Unc5C in situ hybridization shows additional ectopic expression of Unc5C within cells electroporated with Satb2/Ski apart from the expression in the subplate seen in the contralateral hemisphere or in the control electroporation. (c) Unc5C mRNA expression with respect to Satb2 and Ctip2-expressing cells through immunofluorescence/fluorescence ISH in P0 wild-type cortex. High magnification of P0 wild-type cortex shows that Unc5C is expressed primarily in the upper layers but also in a subset of cells in the deep layers—layer V (boxed regions) Unc5C mRNA expression coincides with the expression of Satb2 and is excluded from Ctip2-expressing cells. Layer V expression of Unc5C is restricted to cells expressing Satb2 (arrows) and absent in Ctip2-positive cells (double arrowheads). Scale bar, 100 μm, unless specified differently in the figure.
