Figure 8: Satb2 electroporation in Satb2−/− cortex partially rescues the CC.
From: Unc5C and DCC act downstream of Ctip2 and Satb2 and contribute to corpus callosum formation
In utero electroporation of plasmids encoding for Satb2 and EGFP in wild-type and Satb2−/− embryos at E12.5, harvested at E18.5 followed by immunohistochemical staining for the neuronal adhesion molecule L1, show a partial rescue of the CC in Satb2−/− mutants. Alternatively, sections were counter stained with the far-red nuclear marker Draq5. Arrows indicate fibres crossing the midline. Scale bar, 450 μm for low-magnification images and 100 μm for high-magnification images.
