Figure 8: Leukocyte rolling and adhesion is impaired in anxA8 KO mice. | Nature Communications

Figure 8: Leukocyte rolling and adhesion is impaired in anxA8 KO mice.

From: Annexin A8 controls leukocyte recruitment to activated endothelial cells via cell surface delivery of CD63

Figure 8

(a) Cremaster muscles were prepared from anxA8 KO mice (fl/fl;pgk-Cre) as well as from heterozygous littermates (+/fl;pgk-Cre). The number of adherent leukocytes (b), leukocyte rolling flux (c) and corresponding rolling velocity (d) were determined by transillumination intravital microscopy of blood vessels in the Cremaster. Scale bars, 10 μm. Leukocyte numbers were determined from at least 18 movies (four individuals per condition) of different postcapillary venules (b,c). (d) Rolling velocities of at least 60 rolling leukocytes were analysed in the respective intravital movies. Statistical analysis was performed by unpaired Student’s t-test; ***P<0.001. (e) Murine whole-blood samples from anxA8 KO mice as well as from heterozygous littermates were analysed for PSGL-1 surface expression on leukocytes by flow cytometry. Bars represent means±s.e.m. from at least 900 leukocytes per mouse. Statistical analysis was performed by unpaired Student’s t-test. P>0.05=NS.

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