Figure 3: STAT3 controls the production of IL-22 by CD4+ T cells stimulated with IL-21. | Nature Communications

Figure 3: STAT3 controls the production of IL-22 by CD4+ T cells stimulated with IL-21.

From: IL-21 induces IL-22 production in CD4+ T cells

Figure 3

(a,b) Naïve WT CD4+ T cells were activated in the presence of IL-21 or IL-6 and TGFβ1. (a) FACS analysis of phosphorylated STAT3. Mean fluorescence intensity (MFI) of phosphorylated STAT3 normalized to total STAT3. (b) qPCR analysis of stat3 expression. (c–f) Naïve WT and STAT3-deficient CD4+ T cells were activated in the presence of IL-21 or IL-6 and TGFβ1. (c) Effect of STAT3 deficiency on IL-22 and IL-17 production. (d) Effect of STAT3 deficiency on il21 expression. (e,f) Effect of STAT3 deficiency on ahr and rorc expression (e) and the receptors il1r and il23r (f). (g) Induction of stat3 by IL-1β, IL-21, IL-23, IL-1β and IL-21 or IL-21 and IL-23. mRNA expression is shown relative to gapdh. Results are representative of two to three independent experiments. *P<0.05, **P<0.01 and ***P<0.001 (one-way ANOVA and Student’s t-test).

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