Figure 3: Venomics.

(a) Coomassie-blue-stained SDS-gel of venom from tarantula and velvet spider, respectively, (Supplementary Fig. 16). The major protein(s) in the bands are: T1, membrane venom metalloendopeptidase-a; T2, putative cysteine-rich venom protease; T3, genicutoxin A1; V1, membrane venom metalloendopeptidase and venom aminopeptidase-a; V2, venom pancreatic-like triacylglycerol lipase-a and c; V3, cysteine-rich venom protease-a; V4, venom phospholipase A2-a; and V5, S.m. Sp2b. In addition to S.m. Sp2b, the V5 band also contains several protoxins. The composition of the visual blue bands was specifically interrogated using the relevant LC-MS/MS analyses of bands from the gels shown in Supplementary Figs 4 and 5. The data are a sub-fraction of the data shown in Supplementary Data 1 and 2, where the merged result of LC-MS/MS analyses of gel bands from a complete gel lane is presented. (b) The table summarizes quantitative analyses of venom proteins, excluding protoxins (mainly present in the lower bands on the gel in Fig. 3a). The reason to exclude the protoxins from this analysis is described in the Supplementary Note 2. The proteases are shown in red, the lipases in grey and the other proteins in shades of blue. Numbers in parentheses refer to the number of variants of the particular protein that were quantified. Individual proteins constituting <1% of the venom protein content are not included. These quantitative LC-MS/MS analyses are based on a gel-free approach and extracted ion chromatography (XIC). The table is an extract of the full quantitative analyses (Supplementary Data 7 and 8). (c) The genomic localization of the protoxin families in the velvet spider, the stegotoxins, is depicted. The letters (A–F) indicate the family, based on sequence similarities, and the numbers distinguish between the different toxins in the same family (Supplementary Note 2). The ‘A-homologue’ refers to a sequence homologous with the A-family of toxins, but without proteomic support. The arrows indicate the direction of transcription. Introns are shown in red and coding sequences in black. In the A-family cluster, two non-related predicted protein-coding genes are present. These are shown as blue rectangles. The figure shows that toxins with sequence similarities cluster on the genome.