Figure 2: klf-1 is required and specific for the extension of lifespan by dietary restriction. | Nature Communications

Figure 2: klf-1 is required and specific for the extension of lifespan by dietary restriction.

From: A Krüppel-like factor downstream of the E3 ligase WWP-1 mediates dietary-restriction-induced longevity in Caenorhabditis elegans

Figure 2

Lifespan curves represent combined data from multiple independent experiments. Lifespan values are given in Supplementary Table 2. (a,b) Lifespan analysis of eat-2(ad1116) mutant worms (a) or N2 worms (b) fed bacteria expressing klf-1 dsRNA only during adulthood or control vector. (c) Schematic representation of liquid culture bacterial dilution experiments with RNAi. (d) Lifespan analysis of N2 worms grown in diet restricted (DR) or ad libitum (AL) E. coli (OP50) concentrations in liquid culture from day 3 adulthood after feeding bacteria expressing klf-1 dsRNA or control vector on plates. (e) Lifespan analysis of daf-2(e1368) mutant animals fed klf-1 dsRNA only during adulthood or control vector. RNAi depletion of klf-2 or klf-3 during adulthood had no effect on daf-2(e1368) lifespan (data not shown). (f,g) Lifespan analysis of eat-2(ad1116) mutant worms fed bacteria expressing klf-2 (f), klf-3 (g) dsRNA only during adulthood or control vector.

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