Figure 1: Expression of Ebf3 at sites of osteogenesis and muscle formation.
From: Ebf factors and MyoD cooperate to regulate muscle relaxation via Atp2a1
(a–c) Staining for β-galactosidase in cryosections of E16.5 Ebf3+/+ (a), Ebf3+/− (b) and Ebf3−/− (c) embryos. Ebf3-β-gal-positive cells can be detected in neuronal, olfactory and adipocytic tissues and at sites of osteogenesis and muscle formation. Staining in the lumen of the duodenum is background. (d–f) Magnification of Ebf3-β-gal staining in the area surrounding parts of the diaphragm in Ebf3+/+ (d), Ebf3+/− (e) and Ebf3−/− (f) embryos as indicated by black boxes in a–c. The diaphragm in this magnification is surrounded by the lung and liver tissue as indicated. Bar, 100 μm. (g) Quantitative PCR analysis of the expression of Ebf3 in various tissues of wild-type E18.5 embryos (left) or adult animals (right). The pro-B cell line Ba/F3 was used as a negative control and set to 1. n=3; error bars=s.d. Significance is calculated in comparison with BaF/3. **P<0.01, ***P<0.001. (h) Single-cell suspensions from the diaphragm of E18.5 Ebf3+/− embryos were sorted into Ebf3-β-gal-positive and -negative cells and subjected to expression analysis of the indicated marker genes for slow (MyHC-Iβ) and fast (MyHC-IIA, MyHC-IIB, MyHC-IIX/D) twitch muscle types by qPCR. n=3; error bars=s.d., *P<0.05 **P<0.01.
