Figure 2: Deletion of Ebf3 leads to respiratory failure, postnatal death and alterations in muscle morphology.
From: Ebf factors and MyoD cooperate to regulate muscle relaxation via Atp2a1
(a) Frequencies of Ebf3 wild-type and mutant genotypes before and after birth. No Ebf3-deficient mice can be detected 12 h after birth; E18.5: n=415, D0.5: n=58. (b) Comparison of wild-type (left), Ebf3-heterozygous (middle) and Ebf3-deficient (right) newborn mice shortly after birth. Length is indicated in centimetres. (c–e) Histological analysis of longitudinal sections of the lung of wild-type (upper row) and Ebf3-deficient (lower row) newborn mice stained with haematoxylin/eosin. d and e are increasing magnifications as indicated by the insets. Bar, c: 200 μm; d: 100 μm; e: 50 μm. (f) Alcian blue/alizarin red staining of cartilage and bone of wild-type or Ebf3−/− skeletons at E18.5. (g,h) Cross-sections of diaphragm muscle fibres of newborn wild-type (g) or Ebf3-deficient (h) mice stained with haematoxylin/eosin. Lower panels are higher magnifications as indicated by the inset in the upper panels. Bars, upper panels: 50 μm; lower panels: 20 μm.
