Figure 1: Iqcg morphants show deficient definitive haematopoiesis and reactivation of CaMKIV rescues the c-myb expression. | Nature Communications

Figure 1: Iqcg morphants show deficient definitive haematopoiesis and reactivation of CaMKIV rescues the c-myb expression.

From: Functional and molecular features of the calmodulin-interacting protein IQCG required for haematopoiesis in zebrafish

Figure 1

(a) Iqcg morphants show disordered CHTs, diminished HSC population and multilineage haematopoiesis. Tails at 4.5 d.p.f. were analysed with control (5 mm for five mismatched nucleotides) and test (MO1) morpholinos. The red and blue lines were drawn to indicate the site of ca and cv, respectively. (b) Whole-mount mRNA in situ hybridization (WISH) of flk1 expression in controls and iqcg morphants at 36 h.p.f. (c) WISH of c-myb expression in controls and iqcg morphants at 48 h.p.f. (d) The CHT regions of Tg(c-myb:eGFP) injected with iqcg MO1 or iqcg 5 mm MO were analysed. (e–j) WISH of HSC or multilineage markers in controls and iqcg morphants. (k) O-dianisidine staining of haemoglobin in controls and iqcg morphants at 5 d.p.f. (l) Whole-mount immunofluorescence of p-CaMKIV in controls and iqcg morphants at 36 h.p.f. Anatomical landmarks: caudal haematopoietic tissue (CHT), caudal aorta (ca), cardinal vein (cv), cloaca (c), notochord (ntc) and somite muscles (s). The blue, green and red arrows indicate the thymus, kidney and heart, respectively. All the left panels are control embryos, whereas all the right panels are iqcg morphants. (m–r) Reactivation of CaMKIV rescues the c-myb expression in iqcg morphants. Rescue experiments with in vitro synthesized WT CaMKIV (o), CACaMKIV (p), DNCaMKIV (K58E) (q) or DNCaMKIV (T183A) (r) mRNAs. WISH of c-myb expression in the indicated embryos at 36 h.p.f. Right panels are enlarged views of the AGM regions corresponding to the left panels.

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