Figure 3: Examination of cellular defects in iqcg-deficient c-myb+ cells. | Nature Communications

Figure 3: Examination of cellular defects in iqcg-deficient c-myb+ cells.

From: Functional and molecular features of the calmodulin-interacting protein IQCG required for haematopoiesis in zebrafish

Figure 3

(a–g) Double staining of c-myb and pH3 in the CHT of controls (a), iqcg morphants (b) and camkIV morphants (c). Rescue experiments with in vitro synthesized WT CaMKIV (d), CACaMKIV (e), DNCaMKIV (K58E) (f) or DNCaMKIV (T183A) (g) mRNAs. Arrows indicate c-myb and pH3 double-positive cells. Scale bar, 50 μm. (h) Histogram represents the percentage of pH3+/c-myb+ cells in each experimental group. At least three independent experiments were performed. Results are expressed as means±s.e.m. GraphPad Prism software was used for statistical analysis. Student’s t-test was used for determining the significance of the results. P<0.05 was considered statistically significant.

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