Figure 2: p53LZ1 and p53LZ2 interact with both HDM2 and HDMX in vitro. | Nature Communications

Figure 2: p53LZ1 and p53LZ2 interact with both HDM2 and HDMX in vitro.

From: Protein grafting of p53TAD onto a leucine zipper scaffold generates a potent HDM dual inhibitor

Figure 2

(a) In vitro GST pull-down assay of designed p53LZs against both HDM2 and HDMX N-terminal domains (HDM2NTD and HDMXNTD). GST or GST-tagged proteins were incubated with HDM2NTD or HDMXNTD and then the protein mixtures were precipitated with glutathione resin after washing to remove unbound proteins. (b) Gel filtration analysis on GST-p53LZ2 in the absence and presence of HDM2NTD or HDMXNTD. The retention volumes and SDS–PAGE results are indicated. (c) Multi-angle light scattering (MALS) measurement of p53LZ2 bound to HDM2NTD or HDMXNTD showing the relative light scattering signal as a function of elution volume. The measured molecular mass of each peak is shown in blue.

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