Figure 3: p53LZ2 exhibits high-affinity binding to both HDM2 and HDMX, relative to WT p53 in vitro. | Nature Communications

Figure 3: p53LZ2 exhibits high-affinity binding to both HDM2 and HDMX, relative to WT p53 in vitro.

From: Protein grafting of p53TAD onto a leucine zipper scaffold generates a potent HDM dual inhibitor

Figure 3

GST or GST proteins were used in ELISA assays of (a) MBP-FL HDM2, (b) MBP-HDM2NTD and (c) MBP-HDMXNTD binding. GST-p53LZ2 harbouring five grafted residues, G10, F14, S15, W18 and L21, showed significantly higher binding affinities to HDM2 and HDMX in comparison with WT p53TAD (residues 1–72) and GST-p53LZ1. All data are presented as mean±s.d. of three independent experiments. (d) ITC titration and fitting curves for p53LZ2 binding to HDM2NTD or HDMXNTD. The numbers show the fitting of the data, with N (stoichiometry), KD (dissociation constant), ΔH (enthalpy change) and −TΔS (entropy change).

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