Figure 6: The expression pattern of upregulated PHI-base genes and genes for secreted proteins and secondary metabolism during U. virens infection.

(a) A total of 213 genes involved in pathogen–host interactions were upregulated at 6 h, 24 h and 48 h post inoculation (h.p.i.). (b) A total of 116 genes encoding predicted secreted proteins including 28 putative effectors were upregulated at 6 h.p.i., 24 h.p.i. and 48 h.p.i. Red dots indicate putative effector proteins. (c) A total of 65 genes involved in secondary metabolic processes were transcriptionally induced at 6 h.p.i., 24 h.p.i. and 48 h.p.i. Blue dots indicate predicted PKS/PKS-like proteins; yellow dots indicate predicted NRPS/NRPS-like proteins. These genes are significantly enriched in the upregulated genes during early stages of U. virens infection. Hierarchical clustering analysis was performed for these transcripts at 6 h.p.i., 24 h.p.i. and 48 h.p.i. Each column represents the fold change in transcript levels in planta at the indicated times, relative to the levels in vitro. The vertical dimension represents genes that exhibited changes in transcript level (cutoff: |log₂[fold change]|⩾1 and false discovery rate≤0.001). The colour scale indicates transcript abundance relative to the mycelium before inoculation: red, increase in relative transcript abundance; blue, decrease in relative transcript abundance.