Figure 4: LNP-formulated siRNA induces efficient knockdown in nodules of spontaneous hepatocellular carcinoma. | Nature Communications

Figure 4: LNP-formulated siRNA induces efficient knockdown in nodules of spontaneous hepatocellular carcinoma.

From: Nanoparticle-formulated siRNA targeting integrins inhibits hepatocellular carcinoma progression in mice

Figure 4

(a,b) Western blot analysis of alpha-fetoprotein levels in serum samples collected form animals 10 weeks after tumour induction. Afp levels compared pre-dosing, upper panel, and 3 days after siRNA injection, lower panel (n=3, mean±s.e.m., P-levels—comparison by the Turkey post hoc test). (c) Dose response of Itgb1 silencing in tumour nodules and adjacent liver tissue analysed by qPCR, tissues collected 1 day after injection (n=3, mean±s.e.m., comparison by the Turkey post hoc test). (d) Western blot analysis of Itgb1 levels in livers bearing tumours treated with si-Control of si-Itgb1 for 2 weeks. (e) Quantification of western blot, (d) n=4, mean±s.e.m., comparison by the Student t-test. (f) Immunofluorescent staining of Itgb1 in tumour tissue (after 2 weeks of treatment with si-Itgb1). Tumour nodes countered with white dashed line. Red arrowheads indicate Itgb1 expression on tumour cells. Scale bar, 100 μm. See Supplementary Figs 11 and 12.

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