Figure 6: Effect of Itgb1 silencing on proliferation and survival of tumour cells in vivo. | Nature Communications

Figure 6: Effect of Itgb1 silencing on proliferation and survival of tumour cells in vivo.

From: Nanoparticle-formulated siRNA targeting integrins inhibits hepatocellular carcinoma progression in mice

Figure 6

(a) Ki67 staining was used to analyse proliferation of tumour cells, brown nuclei indicate positive cells (arrowheads). Scale bar, 200 μm. (b) Analysis of cell death in tumour nodules by terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL), red nuclei represent TUNEL-positive cells. Scale bar, 200 μm. (c,d) Analysis of expression of cleaved caspase 3 (p17) in livers bearing tumours and treated with either control or Itgb1 siRNA (n=3–4, means±s.e.m., comparison by the Turkey post hoc test). For Itgb1 knockdown validation see Fig. 8a, (e) qPCR analysis of expression of Cclna1, Cclnd1, Gas2l3, Ckap2, Cdkn3, Gadd45b, Birc2 and Axin2 (n=5–6, mean±s.e.m., comparison by the Turkey post hoc test). NTC, non-tumour control mice.

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