Figure 5: Generation of mice lacking Tlr4 in myeloid cells. | Nature Communications

Figure 5: Generation of mice lacking Tlr4 in myeloid cells.

From: Hepatocyte Toll-like receptor 4 regulates obesity-induced inflammation and insulin resistance

Figure 5

(a) PCR analysis of genomic DNA isolated from the indicated tissues of 6-week-old Tlr4ΔmΦ mice. (b) qPCR analysis of Tlr4 mRNA expression in peritoneal macrophage (PM) isolated from chow-fed mice. (c) LPS (1 mg kg−1 body weight) was injected intraperitoneally to chow-fed mice. Blood was collected 1.5 h after LPS administration and plasma Tnfα levels were measured (n=5–6). (d) Levels are presented as a percentage relative to Tlr4fl/fl levels. *P<0.05, ***P<0.001, compared between Tlr4ΔmΦ mice and Tlr4fl/fl mice (Student’s t-test). All data are presented as means±s.e.m.

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