Figure 6: Macrophage Tlr4 deficiency does not protect mice from diet-induced obesity or insulin resistance.

(a) Body weight curves in Tlr4^fl/fl and Tlr4^ΔmΦ mice on a chow (n=9–11) or HFD (n=15) for 12 weeks. (b,c) Blood glucose (b) and plasma insulin (c) concentrations (n=6–8) in Tlr4^fl/fl and Tlr4^ΔmΦ mice after 8 weeks of diet feeding under either fed or overnight fasted states. (d) Glucose tolerance test (GTT, 1.5 mg g⁻¹ BW, n=5–9 for chow-fed mice; n=8 for mice on HFD) in overnight fasted mice. (e) Glucose-stimulated insulin secretion in overnight fasted HFD-fed mice. Glucose (1.5 mg g⁻¹ BW) were injected intraperitoneally and plasma insulin concentrations were measured at the indicated time points (n=8–9). (f) Insulin tolerance test (ITT, 1.5 mU g⁻¹ BW, n=5–8 for chow-fed mice; n=11–13 for mice on HFD) in mice fasted for 5 h. *P<0.05, compared between Tlr4^fl/fl and Tlr4^ΔmΦ mice on the same diet; ^#P<0.05, ^##P<0.01, compared between different diets for mice of the same genotype (Student’s t-test). All data are presented as means±s.e.m.