Figure 5: The PI3Kα/Akt axis is important for R-Ras2-mediated in vivo tumorigenesis. | Nature Communications

Figure 5: The PI3Kα/Akt axis is important for R-Ras2-mediated in vivo tumorigenesis.

From: Contribution of the R-Ras2 GTP-binding protein to primary breast tumorigenesis and late-stage metastatic disease

Figure 5

(a) Representative example of tumours derived from indicated MDA-MB-231-Luc cells. (b) Growth rates of tumours derived from indicated MDA-MB-231-Luc cells (n=6). *P≤0.01; ***P≤0.001 relative to values obtained in control cells at final experimental time point; t-test. (c) Representative example of tumours formed by indicated 4T1 cells. (d) Growth rates of tumours derived from indicated 4T1 cells (n=6). *P≤0.01; ***P≤0.001 relative to values obtained in either control cells or the indicated experimental pairs (in brackets) at final experimental time point; t-test. (e) Phosphorylation and expression status of AKT and FOXO1/3a in indicated, serum-starved MDA-MB-231-Luc cells (top). A long (top panel) and short (SE, second panel from top) exposure is included for the anti-pS473 AKT immunoblotting. (f) Phosphorylation and expression status of Akt in indicated, exponentially growing 4T1 cells. (g) Representative example of tumours formed by indicated MDA-MB-231-Luc cells (top). (h) Growth rates of tumours formed by indicated MDA-MB-231-Luc cells. *P≤0.01; ***P≤0.001 relative to values obtained in control cells or indicated experimental pairs (brackets) at final experimental time points (n=10, 10 and 4 for MDA-Control, MDA+R-RAS2G23V and MDA+R-RAS2G23V+KDPIK3CA, respectively); t-test. (i) In vitro proliferation of indicated MDA-MB-231-Luc cell lines (n=3). In panels b, d, h and i, experimental values are given as mean±s.e.m.

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