Figure 8: R-Ras2 is important for Her2-mediated tumorigenesis. | Nature Communications

Figure 8: R-Ras2 is important for Her2-mediated tumorigenesis.

From: Contribution of the R-Ras2 GTP-binding protein to primary breast tumorigenesis and late-stage metastatic disease

Figure 8

(a) Representative mammary fat pads from mice of indicated ages (top) and genotypes (left). Scale bar, 500 μm. Insets show enlarged views of boxed areas (scale bar, 50 μm). *Lymph node. (b) Quantification of area (left), maximal length (middle) and terminal end bud (TEB) numbers (right) of glands from mice of indicated ages and genotypes (n=5). *P≤0.05; **P≤0.01; ***P≤0.001 relative to appropriate control; t-test. (c) Phosphorylation and expression status of indicated proteins in EGF-stimulated MECs obtained from 6-month-old female mice. (d) Tumour-free survival of MMTV-Her2tg;Rras2+/+ (n=20) and MMTV-Her2tg;Rras2−/− (n=22) mice. ***P≤0.001 relative to controls at the final experimental time point; t-test. (e) Tumour per mouse average in MMTV-Her2tg;Rras2+/+ (n=20) and MMTV-Her2tg;Rras2−/− (n=22) mice. ***P≤0.001 relative to controls at the final experimental time point; t-test. (f) Average size of tumours developed in MMTV-Her2tg;Rras2+/+ (n=20) and MMTV-Her2tg;Rras2−/− (n=22) mice. **P≤0.01 relative to controls at the final experimental time point; t-test. In b, d, e and f, values are given as mean±s.e.m. (g) Mean percentage of tumour-bearing mice with lung metastases. (h) Representative whole mount-stained (top panels; scale bar, 500 μm) and haematoxylin/eosin-stained (bottom panels; scale bar, 100 μm) samples from inguinal, ‘non-tumorigenic’ mammary glands from indicated mice (top). White asterisks, inguinal lymph nodes; white arrowhead, example of a small adenocarcinoma; black asterisk, example of cystic dilation; black arrowheads, examples of hyperplasic lobulo-alveolar units. (i) Quantification of hyperplasic parameters in ‘non-tumorigenic’ mammary glands from indicated mice. Grade 0, no hyperplasia; grade 1, glands with ductal ramifications, lobulo-alveolar terminal units or cystic dilatations; grade 2, glands showing at least two of those hyperplasic parameters. n=15 and 21 control, and MMTV-Her2tg;Rras2−/− mice, respectively. (j) Phosphorylation and expression status of indicated proteins (left) in tumour samples (top) obtained from control and MMTV-Her2tg;Rras2−/− mice.

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