Figure 3: Diet structures the intestinal microbiome of Macaca fuscata at the genus level.

Intestinal samples were collected from M. fuscata maintained on an isocaloric diet consisting of either 13% fat (control diet) or 36% fat (high fat diet). DNA was isolated from intestinal contents, and 16S rDNA pyrosequencing (454 FLXtitanium) was performed. Sequences were analysed using QIIME software. (a) Cladogram obtained from LEfSe analysis of 16S sequences. Green shaded areas indicate bacteria with a higher abundance in control diet fed animals (CTD), while blue shaded areas indicate bacteria with a higher abundance in animals fed a high-fat diet (HFD). (b) Relative abundance of bacterial genera between animals fed a control diet (CTD, green bars) or a high-fat diet (HFD, blue bars). Box plots demonstrate the distribution of data with the line in the middle of the box representing the median. The left of the median is indicative of the third quartile and the right is indicative of the first quartile. Lines to the right of the box are indicative of the 1.5 interquartile range of the lower quartile and lines to the left are indicative of the 1.5 interquartile range of the upper quartile. Dots represent outliers.