Figure 5: Exposure to a maternal high-fat diet persistently alters the offsprings intestinal microbiome with significant depletion of Proteobacteria, including Campylobacter spp. | Nature Communications

Figure 5: Exposure to a maternal high-fat diet persistently alters the offsprings intestinal microbiome with significant depletion of Proteobacteria, including Campylobacter spp.

From: High-fat maternal diet during pregnancy persistently alters the offspring microbiome in a primate model

Figure 5

M. fuscata offspring were vaginally delivered to mothers consuming a control (CTD) or high-fat diet (HFD). Infants consumed the maternal diet until weaning when they were either maintained on the maternal diet (control cohort) or switched to the opposing diet (crossover cohort). At 1 year of age, juveniles were killed and DNA was isolated from intestinal samples. DNA was subjected to 16S rDNA pyrosequencing, and sequences were analysed using QIIME. Juveniles are designated based on the maternal/post-wean diets; that is, CTD/HFD indicates a juvenile that was born to a dam consuming a control diet and switched to a high fat diet post-weaning. Red colour represents juveniles on a control diet/control diet (CTD/CTD), dark green indicates juveniles on a high-fat diet/high fat diet (HFD/HFD), orange indicates juveniles on a high-fat diet/control diet (HFD/CTD) and light green indicates juveniles on a control diet/high-fat diet (CTD/HFD). (a) Relative abundance of bacterial genera in intestinal samples of juveniles maintained on a control diet post-weaning. Juveniles are designated based on the maternal/post-wean diet. Box plots show the distribution of data, with the line in the middle of the box representing the median. The left of the median is indicative of the third quartile and the right is indicative of the first quartile. Lines to the right of the box are indicative of the 1.5 interquartile range of the lower quartile and lines to the left are indicative of the 1.5 interquartile range of the upper quartile. Dots represent outliers. The asterisk by Campylobacter indicates a statistically significant difference (P<0.05 by t-test) between CTD/CTD and HFD/CTD juveniles. (b) Graph representing the most abundant bacterial genera present in intestinal samples of differential juvenile cohorts. Juveniles are grouped based on maternal/post-wean diet as indicated by the line below the chart. Phylogenetic tree designates the genus represented by each colour.

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