Figure 8: Exposure to a high-fat diet diminishes the presence of Campylobacter. | Nature Communications

Figure 8: Exposure to a high-fat diet diminishes the presence of Campylobacter.

From: High-fat maternal diet during pregnancy persistently alters the offspring microbiome in a primate model

Figure 8

M. fuscata was vaginally birthed to mothers consuming a control or high-fat diet. Infants consumed the maternal diet until weaning when they were either maintained on the maternal diet (control cohort) or switched to the opposing diet (crossover cohort). At 1 year of age, animals were killed and DNA was isolated from the stool. DNA was subjected to PCR amplification for both universal 16S rRNA and for Campylobacter 16S rRNA genes. The number of juveniles (n) in each cohort is indicated in parenthesis in the figure legend. (a) Quantitative real-time PCR (qPCR) analysis of stool isolated from juvenile cohorts designated by maternal/post-wean diet. DNA was amplified for universal and Campylobacter 16S rRNA genes using both TaqMan and SYBR qPCR assays. The presence of Campylobacter 16S rRNA was normalized to total universal 16S rRNA presence to provide relative abundance. Each qPCR assay (TaqMan or SYBR) was repeated in three individual assays. Results were pooled and statistical analysis was performed using a one-way ANOVA (**P<0.05). Line represents the mean. (b) PCR amplification of Campylobacter 16S rDNA (top, 812 bp) or universal 16S rDNA (bottom, 462 bp). PCR products were run on a 3% agarose gel containing ethidium bromide. Full gel can be seen in Supplementary Fig. 3.

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