Figure 4: FACS analysis of digested LV-iEC and MIP-iEC grafts show differences in immune response elicited in syngeneic recipients. | Nature Communications

Figure 4: FACS analysis of digested LV-iEC and MIP-iEC grafts show differences in immune response elicited in syngeneic recipients.

From: Transplanted terminally differentiated induced pluripotent stem cells are accepted by immune mechanisms similar to self-tolerance

Figure 4

(a) Among the leukocytes infiltrating MIP-iEC and LV-iEC grafts, macrophages (Mac) with either suppressor (CD3−CD11b+CD11c−Gr1+) or activated (CD3−CD11b+CD11c+MHC-II+Gr1−) phenotype were present at similar proportions. MIP-iEC grafts had a significantly higher prevalence of neutrophils (CD3−CD11b−CD11c−Gr1+) compared with LV-iEC. (b,c) A higher percentage of CD8+ and CD4+ T cells infiltrating MIP-iEC grafts expressed CD44 effector memory marker compared with the ones infiltrating LV-iEC grafts. (d) CD8+ T cells infiltrating MIP-iEC grafts had a significantly higher expression of granzyme-B (Granz-B) compared with the ones infiltrating LV-iEC grafts. The percentage of FoxP3+ -expressing CD4+ T cells infiltrating the MIP-iEC and LV-iEC grafts was indistinguishable. Data are representative of mean±s.e.m. of 4–5 animals per group from one experiment. Significance of differences between series of results was assessed using two-tailed unpaired Student’s t-test; *P<0.05.

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