Figure 5: iECs elicited activation of a tolerogenic transcriptome network. | Nature Communications

Figure 5: iECs elicited activation of a tolerogenic transcriptome network.

From: Transplanted terminally differentiated induced pluripotent stem cells are accepted by immune mechanisms similar to self-tolerance

Figure 5

(a) Hierarchical clustering revealed a group of nine genes (right panel) consistently upregulated in iEC grafts and with similar expression levels. These genes are known members of a pathway involved in regulation of IL-10. Data are representative of three animals per group. (b) Fold-change in gene expression relative to sham grafts confirming the pro-tolerogenic phenotype of iEC and AEC grafts. Data are representative of mean±s.e.m. of 3–4 animals per group from three independent experiments. Significance of differences between series of results was assessed using analysis of variance with Tukey for multiple comparisons. (c) Immunofluorescence staining demonstrating IL-10 abundance in iPSC, iEC and AEC grafts. Quantification of IL-10 expression was performed in five random fields from each graft type ( × 40 magnitude). Data are representative of mean±s.e.m. of a minimum of 1,000 cells from five randomly selected fields. **P<0.01, ***P<0.001.

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