Figure 6: The Aβ level is reduced in brains of ILEI-Tg mice. | Nature Communications

Figure 6: The Aβ level is reduced in brains of ILEI-Tg mice.

From: The FAM3 superfamily member ILEI ameliorates Alzheimer’s disease-like pathology by destabilizing the penultimate amyloid-β precursor

Figure 6

(a) Immunostaining shows ILEI expression in neurons of the cerebral cortex and hippocampus of wild-type and ILEI-Tg mouse brains (8 months old). Scale bars, 200 μm (left), 500 μm (centre), 20 μm (right). (b) Immunoblots for ILEI and Notch in brains of ILEI-Tg mice and non-Tg littermates (6 months old). β-Actin was used as a loading control. FL, full-length; SF, secreted form. (c) Immunoblots for APP-CTFs in brains of ILEI-Tg mice and non-Tg littermates (6–8 months old, n=6 per genotype). The graph shows the relative intensity of APP-CTFs normalized to β-actin (n=6, mean±s.d.). **P<0.01 versus non-Tg mice by Student’s t-test. (d) Brain Aβ levels in non-Tg and ILEI-Tg mice (10 months old, n=6 per genotype). Aβ40 (black bars) and Aβ42 (grey bars) in Tris-buffered saline-soluble and -insoluble fractions of brain homogenates were measured using ELISA assays (n=6, mean±s.d.). *P<0.05 and **P<0.01 versus non-Tg by Student’s t-test.

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