Figure 2: Anisotropy measurement.
From: In vivo imaging of specific drug–target binding at subcellular resolution

Me5–BODIPY anisotropy dependence on viscosity, as measured in glycerol with MFAM. Measurements are obtained from two-photon images of sample drops of Me5–BODIPY and calculating the anisotropy of each pixel. Average±s.d. (n=6), fitted curve added for trend visualization.