Figure 7: Imaging of AZD2281–BODIPY FL target engagement in a live mouse.
From: In vivo imaging of specific drug–target binding at subcellular resolution
(a) In vivo fluorescence image of injected fluorescent microspheres (pink) in the vascularized (green) tissue fascia of a mouse DSC. Scale bar, 50 μm. (b) Anisotropy of the injected fluorescent microspheres as a function of depth within the tissue fascia. Each point corresponds to a single bead measurement. (c) Confocal fluorescence image of HT1080 H2B mApple cells (red) in a mouse DSC. After 1–2 weeks, the tumour area is highly vascularized and, upon intravenous injection, perfused with AZD2281–BODIPY FL (green). The white square indicates the imaged area in (d). Scale bar, 100 μm. (d) In vivo anisotropy (top) and fluorescence (bottom) images of AZD2281–BODIPY FL following intravenous infusion (left) and 34 min later (right). Scale bar, 20 μm (e) Overall image intensity (black), nuclear intensity (grey) and nuclear anisotropy (unfilled, striped) as measured from the images in (d). Nuclear intensity and anisotropy values are average±s.e. (n=90 for image t1, n=102 for image t1+34 min). Fluorescence intensity refers to the sum of both perpendicular and parallel channels.
