Figure 2: Global reduction of protein levels in the presence of cis-antisense.
From: A global non-coding RNA system modulates fission yeast protein levels in response to stress
Box plots represent change in protein expression in WT (a) and atf21Δ (b) for which sense exceeds antisense (grey) and where antisense exceeds sense (red) in 140 sense–antisense pairs involving ncRNA. All data were normalized relative to t=0.Transcript levels are the mean of three biological replicates; protein expressions are the mean of five technical replicates. Whiskers correspond to the largest or smallest observation that falls within a distance of 1.5 times the interquartile range from the nearest hinge. P-value was calculated using t-test. (c) GO Slim categories that are significantly enriched in loci exhibiting either a higher level of antisense (AS>S) or of sense (S>AS) in a and b. The number of sense–antisense pairs for which the protein-coding transcripts were annotated to the GO Slim term is enumerated in each cell. Significant instances (hypergeometric test, FDR<5%) are coloured red.
