Figure 5: Osmotic stress-responsive ncRNA SPNCRNA.1164 alters the oxidative stress response.
From: A global non-coding RNA system modulates fission yeast protein levels in response to stress
(a) The SPNCRNA.1164 locus, indicating the regions of the genome that were deleted. Two deletion strains were generated: (i) snr1L—the full-length transcript was deleted, and (ii) snr1S—the 5′-non-overlapping portion of the transcript independent of SPNCRNA.1165 and SPNCRNA.1166/prl6 was deleted. (b) Serial dilution assays reveal resistance to H2O2 (1 mM) and t-BOOH (0.75 mM), but not to sorbitol (1 M) in both snr1L and snr1S strains. The experiment was performed three times and representative results are shown. (c) Transcript levels of the four ATF transcription factors in the WT and snr1L cells following induction of stress by treatment with sorbitol (1 M) at indicated timepoints. (d) The four ATF transcription factors exhibited similar response to t-BOOH (0.75 mM) in both snr1L and snr1S strains. Mean and s.e.m. of three biological replicates are shown in c and d.
