Figure 5: Histone-binding ability of the double chromodomains from CHD family.
From: Structural basis for histone mimicry and hijacking of host proteins by influenza virus protein NS1
(a) Sequence alignment of the double chromodomains for all human CHD members. Secondary structural elements of CHD1 are shown above the sequences. Cyan, CD1; Magenta, CD2. Blue triangles, aromatic residues involved in methyl-lysine recognition in CD1; red triangles, corresponding residues in CD2 to the blue triangle residues in CD1; red star, other key residues for CHD1 binding to the AR(T/S)K motif. (b) Dissociation constants (μM) of the double chromodomains of some CHD proteins to different H3K4 peptides measured by FP. (c) FP binding curves for the PHD2-double chromodomains of CHD3. (b,c) Kd values were calculated from single measurement and errors were estimated by fitting curve.
