Figure 9: Proteosome inhibition offsets CaMKIINtide-induced memory impairment and the decrease in AMPARs. | Nature Communications

Figure 9: Proteosome inhibition offsets CaMKIINtide-induced memory impairment and the decrease in AMPARs.

From: pT305-CaMKII stabilizes a learning-induced increase in AMPA receptors for ongoing memory consolidation after classical conditioning

Figure 9

(a) MG132 co-injected with CaMKIINtide at 6 h post training rescues ALTM. Untreated paired (no injection, N=24), DMSO-injected (Vehicle, N=12), MG132-injected (N=12) and CaMKIINtide+MG132-injected (N=12) all showed significantly higher feeding responses to the CS compared with CaMKIINtide-injected (N=24) and naive (N=24) animals. Asterisks indicate significantly higher feeding responses to the CS compared with CaMKIINtide and naive levels. ANOVA: P<0.001. Tukey’s test: CaMKIINtide-injected versus all other trained groups, P<0.05; CaMKIINtide-injected versus Naive group, P>0.05; CaMKIINtide+MG132-injected group versus CaMKIINtide-injected and Naive group, both P<0.05. (b) MG132 co-injected with CaMKIINtide at 6 h post training rescues GluA1 receptor levels. Samples from untreated paired (no injection, N=4), DMSO-injected (Vehicle, N=8), MG132-injected (N=8) and CaMKIINtide+MG132 injected (N=8) animals all showed significantly higher GluA1 levels compared with CaMKIINtide-injected (N=8) and naive (N=8) animals. Full-length blot is shown in Supplementary Fig. 8. Asterisks indicate significantly higher relative integrated density values compared with CaMKIINtide and naive baseline level, respectively. One-way ANOVA: P<0.006. Tukey's: CaMKIINtide-injected versus all other trained groups, P<0.05. One-sample t-tests: CaMKIINtide-injected versus naive baseline, P>0.05; all other groups versus naive baseline, P<0.05. These experiments were replicated twice. All data presented as means±s.e.m.

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