Figure 1: LncRNA expression is regulated by LPS in human monocytes. | Nature Communications

Figure 1: LncRNA expression is regulated by LPS in human monocytes.

From: Long non-coding RNAs and enhancer RNAs regulate the lipopolysaccharide-induced inflammatory response in human monocytes

Figure 1

(a) Pipeline for predicting lncRNAs from cufflinks assembled transfrags. HumanBodyMap=lincRNAs predicted in ref. 2. Can-lncRNAs=canonical lncRNAs characterized by a low H3K4me1/H3K4me3 ratio. eRNAs characterized by a high H3K4me1/H3K4me3 ratio. (b) Release of CXCL8 and TNFα measured by ELISA shows activation of the innate immune response. Data are the mean±s.e.m. of four independent experiments. Statistical significance was determined using a Student t-test where *P<0.05, **P<0.01 and ***P<0.001. (c) Overlap of lncRNAs in monocytes, Gencode v13 and lncRNAs from the HumanBodyMap catalogues. (d) Volcano plot displaying differentially expressed lncRNAs (n=4 in each group). Differential expression analysis was performed using a test based on the negative binomial distribution implemented in DESeq. LncRNAs that are significant at an FDR<0.05 are displayed in blue. (e) Breakdown of differentially expressed lncRNAs based on positional classifications.

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