Figure 4: Snail1 regulates angiogenic programme in a xenograft transplant model.
From: A Snail1/Notch1 signalling axis controls embryonic vascular development
(a) Representative image of isolated ECs from eGFP+;Snail1fl/fl mice. Scale bar, 50 μm. (b) Adeno-β-gal or Adeno-Cre-infected ECs were stimulated with vehicle or VEGF (100 ng ml−1) for 12 h and subjected to western blot analysis. Results are representative of three or more experiments. (c,d) Whole-mount PECAM-1 (CD 31) staining of the implants after 14 d in vivo. Scale bar, 50 μm. (e,g,i,k) Gross view of implants. Scale bar, 2 mm. (f,h,j,l) Whole-mount PECAM-1 staining of the implants. Scale bar, 100 μm. (m) Quantification of relative vessel length in the implants (n=3 in each group). Data are presented as mean±s.e.m. **, $$, ##P<0.01, analysis of variance test.
