Figure 6: Administration of DAPT partially reverses Snail1 deletion-induced vascular defects.

Confocal analysis of PECAM-1 stained whole-mounted untreated (a,c) or DAPT-treated embryos (b,d). Rectangled area highlights improved vascular remodelling in DAPT-treated Snail1 LOF mutant embryos. Scale bar, 100 μm. (e) Quantification of relative vessel length in yolk sacs from E10.5 untreated and DAPT-treated Snail1 LOF embryos (n=4 each). Data are presented as mean±s.e.m. *P<0.05, **P<0.01, Student’s t-test. (f) qRT–PCR analysis of ECs freshly isolated from untreated or DAPT-treated E10.5 embryos (n=4 each). Data are presented as mean±s.e.m. **P<0.01, Student’s t-test. (g) Quantification of embryo size in a–d above. Allantoises dissected from WT (h,i) and Snail1 LOF mutant (j,k) embryos were cultured in the presence of vehicle (h,j) or 8 μM DAPT (i,k) for 24 h and ECs visualized by whole-mount PECAM-1 staining. Scale bar, 100 μm. (l) Quantification of relative vessel length in allantoises (n=4 each). Data are presented as a mean±s.e.m. **P<0.05, Student’s t-test.