Figure 7: Model-guided creation of de novo synthetic promoters.
From: Design of synthetic yeast promoters via tuning of nucleosome architecture
(a) Two synthetic lead sequences, each containing prescribed transcription factor binding sites and randomized linker sequences, were used for de novo promoter design. (b) Three computationally derived versions of each synthetic promoter were tested, one from an early round of optimization, one from an intermediate round and one from a late round. Psynth1v1 and Psynth1v2 are the result of the sixth round, Psynth1v2 is from the 50th, Psynth1v3 is from the 98th, Psynth2v2 is from the 30th and Psynth2v3 is from the 59th. Expression levels of the redesigned synthetic promoters spanned a nearly 20-fold range and all were functional. Error bars represent s.d. of three biological replicates.
