Figure 2: Structural effects of SOX11 mutations.

(a) Crystal structure of the mouse Sox4 HMG domain bound to DNA. Helices and loops are shown as green ribbons and threads, respectively. DNA is shown as grey sticks. Amino-acid residues at mutation sites are shown coloured red in the space-filling model. In the middle and right images, some of the amino-acid residues involved in the hydrophobic core surrounding mutation points are shown coloured green in the space-filling model. Amino-acid numbering is indicated for mouse Sox4 with that for human SOX11 in parentheses. Hydrogen bonds are shown as black dotted lines. Molecular structures were drawn using PyMOL ( http://www.pymol.org). (b) Free energy changes on the indicated mutations calculated by FoldX software.