Figure 5: hiPSC-derived NR progenitors within the 3D RCs recapitulated the spatiotemporal pattern of NR differentiation in vivo.
From: Generation of three-dimensional retinal tissue with functional photoreceptors from human iPSCs
(a–l) Cells within the RCs differentiated and migrated to their corresponding layers, with ganglion cells (GCs; BRN3-positive, (a–g); TUJ1-positive, h where * indicates a developing nerve-fibre-like layer) appearing first, followed by photoreceptor cells (PRC; OTX2/recoverin-positive, a–c, e–g), amacrine cells (AC; AP2α-positive, i–l) and horizontal cells (HC; PROX1-positive, i–l). (m–p) By W21–W23, RCs presented a well-established outer nuclear layer (REC-positive, m,n) delineated by a developing outer plexiform layer (SV2-positive, n) and containing rod opsin-positive photoreceptors (o) and a developing bipolar cell layer containing bipolar cells (BC; VSX2-positive/MCM2-negative, p) intermingled with the remaining retinal progenitor cells (RPC; VSX2-positive/MCM2-positive, p). (q) Timeline of retinogenesis in hiPSC-derived 3D RCs. Scale bars, 100 μm (a–d); 20 μm (e–p).
