Figure 5: The inactivation gate selectively enters the intracellular vestibule.
From: Quasi-specific access of the potassium channel inactivation gate
(a) Shaker homotetramer E192K D193K mutant. Cartoon (top) and representative current trace (below) in response to a voltage step to +60 mV from a holding potential of −80 mV. Horizontal bar for all panels: 10 ms. Vertical bar: 100 pA. (b) Shaker concatemer having all subunits mutated to E192K 193K. Cartoon (top) and representative current trace (below). Vertical bar: 200 pA. (c) Shaker concatemer having the E192K D193K mutation at only the free N-terminus containing subunit. Cartoon (top) and representative current trace (below). Vertical bar: 100 pA. (d–f), cartoons (top) and representative current traces (below) in response to a voltage step to +60 mV from a holding potential of −80 mV and their corresponding for: Shaker concatemer having the E192K D193K mutation at only subunit 2 (d), Shaker concatemer having the E192K D193K mutation at only subunit 3 (e) and Shaker concatemer having the E192K D193K mutation at only subunit 4 (f). Vertical bars (from left to right): 500, 250 and 250 pA. n=3 for all constructs.
