Figure 1: Rat proprioceptive axons lose responsiveness to Sema3A at embryonic day 14. | Nature Communications

Figure 1: Rat proprioceptive axons lose responsiveness to Sema3A at embryonic day 14.

From: ADAM metalloproteases promote a developmental switch in responsiveness to the axonal repellant Sema3A

Figure 1

DRGs from E13, E14 and E15 rat embryos were embedded in collagen matrix, next to an aggregate of COS-1 cells, either secreting (a,b), or not secreting (c,d), Sema3A. Explants were cultured in the presence of NT3 (a,c), or NGF (b,d), to promote growth of proprioceptive or cutaneous axons, respectively. (e) Scheme illustrating the quantification principals of the assay. For each DRG, axonal length was computationally measured in the range of ±45° around the horizontal axis on both sides of the explant. Total axon length on the DRG side that is proximal (P) to the aggregate was divided by the total axon length on its distal (d) side to obtain a P/D ratio for each explant. Average P/D ratios calculated for proprioceptive and cutaneous axons at the different developmental stages are presented in (f,g), respectively. Results were collected from at least three separate experiments for each condition. n=number of ganglia measured for each developmental stage. Bars represent mean±s.e.m. Mann–Whitney U-test. ***P<0.001, NS, non significant. DRG, dorsal root ganglion.

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