Figure 6: ADAM10/17 double-knockout proprioceptive axons exhibit increased sensitivity to Sema3As. | Nature Communications

Figure 6: ADAM10/17 double-knockout proprioceptive axons exhibit increased sensitivity to Sema3As.

From: ADAM metalloproteases promote a developmental switch in responsiveness to the axonal repellant Sema3A

Figure 6

Wild type and mutant proprioceptive neurons were tested in collagen repulsion assay for sensitivity to Sema3A. DRGs were dissected from wild type, ADAM10 cKO (a), ADAM17 cKO (b) and ADAM10/17 double cKO (c,e) mice embryos at E12.5, or wild type and ADAM10/17 double cKO embryos at E13.5 (d). Explants were embedded in collagen matrix, next to Sema3A-secreting- (a–d) or non-transfected (e) aggregates of COS-1 cells, as indicated. Each droplet of collagen harbored a pair of wild type and mutant DRGs, originating from littermate embryos. Culture media were supplemented with NT3 to support the growth of proprioceptive axons. (f) P/D ratio quantification for wild type and mutant DRGs, cultured in the presence of Sema3A-secreting aggregates. Results were collected from 3 to 6 separate experiments for each condition; n=number of ganglia measured for each condition. Bars represent mean±s.e.m. Mann–Whitney U-test. ***P<0.001; NS, non significant.

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