Figure 3: 3D reconstruction of the single-shot diffraction pattern (Fig. 2a). | Nature Communications

Figure 3: 3D reconstruction of the single-shot diffraction pattern (Fig. 2a).

From: Single-shot three-dimensional structure determination of nanocrystals with femtosecond X-ray free-electron laser pulses

Figure 3

(a–c) Iso-surface renderings of the final 3D reconstruction along the two-, three- and fourfold rotational symmetry, respectively (a threshold of 30% of the maximum density was used to display these images). The insets show 3.3-nm-thick centro-slices of the 3D reconstruction along the corresponding directions. The variation of the electron density inside the nanocrystal is within ±10%, which is mainly due to noise in the single-shot diffraction pattern and the intensity variation inside each XFEL pulse. (d) FSC comparison between two independently reconstructed gold nanocrystals. Based on the FSC=0.5 criterion, a 3D resolution of the reconstructions was estimated to be ~5.5 nm. The iso-surface rendering images as well as those in Figs 4 and 6 are displayed by Amira with interpolation. Scale bar, 30 nm.

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