Figure 6: PtdIns5P localizes to early endosomes with Tiam1 and Rac1.
From: Phosphatidylinositol 5-phosphate regulates invasion through binding and activation of Tiam1
(a) MEF-IpgD were co-transfected with mRFP-Rac1 and Tiam1-HA before induction of IpgD expression. Cells were then serum-starved, fixed and stained for EEA1 and the HA tag. The lower panels (zoom 1 and zoom 2) represent high magnifications of areas 1 and 2 shown on the upper merge panel. On each zoom, the two lines drawn across the cells indicate the path of a 3-pixel-wide linescan (zoom 1: a,b, zoom 2: c,d). Respective line scans are shown below zoom panels. (b) Serum-starved MEF-IpgD were stained for endogenous Rac1, EEA1 and PtdIns5P (using the GST-2xPHD probe). Arrowheads indicate zones of ruffling. Magnifications of areas 1, 2 and 3 shown as boxes in the merged image, as well as their respective linescans (3-pixels wide) are shown at the bottom of the panel. Scale bar, 10 μm.
