Figure 4: Tcf3 induction rescues the defective migration in Stat3-deficient cells.
From: Tcf3 promotes cell migration and wound repair through regulation of lipocalin 2
(a) Primary keratinocytes from Stat3fl/fl (cont) or Stat3fl/fl;K14-Cre (Stat3 cKO) mice were isolated and were transduced with GFP-tagged lentiviral vector expressing tet-inducible Tcf3 or with empty vector control. After the transduced cells were enriched by fluorescent cell sorting, they were grown to confluence and then subjected to a migration assay with or without Dox. Images of keratinocytes 48 h after initiation of the migration assay. Black bar denotes 200 μm. (b) Graph quantifying the relative area the cells migrated normalized over vehicle control. For each sample, over 30 non-overlapping fields were measured at each time point; and each experiment was repeated three times. Data are the mean±s.d. **P<0.001 (Student’s t-test).
