Figure 7: Lcn2 is sufficient to promote cell migration and wound healing.

(a) Migration assays were performed on keratinocytes that were incubated with Lcn2-CM or control (empty vector) CM (cont). Graph quantifying the relative migrated area of keratinocytes treated with the indicated CM. For each sample, over 30 non-overlapping fields were measured at each time point; and each experiment was repeated twice. Data are mean±s.d. **P<0.001. (b) Full-thickness wounds (1 cm²) were created on dorsal skins of wild-type mice (n=4) and were treated daily with topical application of conditioned media (CM) from Lcn2 (n=4) or control (empty vector) (n=5) transduced keratinocytes. Images of the wound sites were taken at the beginning of the wounding experiment and 10 days post wounding, at which point skins were harvested for analysis. (c) Graph quantifying the effect of topical application of Lcn2-CM on wound closure, conditioned media (CM) from Lcn2 (n=4) or control (empty vector) (n=5). Average surface areas of the wounds 10 days post wounding is quantified as a percentage of the initial wound areas. Areas of the wounds were measured with ImageJ software. Data are mean±s.d. *P<0.05 (Student’s t-test).